TruFluor® DO Single-Use Optical
Dissolved Oxygen Sensors

The TruFluor® DO single-use optical dissolved oxygen measurement solution consists of an optical probe with an embedded temperature sensor. A TruFluor® loop consists
of a disposable sheath, an optical reader, and a transmitter (blade or external).

The single-use sheath can be pre-inserted in a disposable bioreactor bag port and irradiated with the bag, in order to both preserve and guarantee the sterile barrier.
All wetted materials of the sheath are USP class VI compliant.

The optical reader utilizes a patented optical design that minimizes photodegradation of the active sensing element. The design has been optimized to provide accurate
in-situ measurement of DO using phase fluorometric detection in real time.

The temperature measurement leverages a 316L stainless steel thermal window embedded in the sheath, and provides accurate results that can be used as a
process variable, or for temperature compensation.

Benefits

  • Minimize contamination, maximize single-use vessel integrity
  • Obtain real-time DO and temperature
  • Collect drift-free data from pre-calibrated sheaths
  • Meet cGMP regulations with lot traceability and loop validators
  • Rely on USP Class VI / gamma radiation resistant wetted materials

Smart Calibration

SmartChips  All Finesse SmartSensors contain embedded SmartChips that hold factory calibration data and are gamma radiation resistant. The SmartChip also holds manufacturing lot and serial numbers that can be part of the batch record if used with the blade transmitter.

SmartReaders  Each optical SmartReader is normalized to a traceable standard at the factory. The validator loop can be used to verify the performance of the measurement loop prior to each batch run for cGMP operation. For the best measurement results, the user can always perform 1-point standardization or a 2-point calibration using an off-line reference analyzer.

SmartReaders Minimize Drift and Photo-Degradation

TruFluor® pH and TruFluor® DO optical smart readers utilize a patented design that minimizes photo-degradation of the active sensing element. The design has been optimized to provide accurate in-situ measurement of pH and dissolved oxygen using phase fluorometric detection in real-time. The temperature measurement leverages a 316L stainless steel thermal window embedded in the sheath, and provides accurate results that can also be used as a process variable.

TruFluor® DO sensors are based on oxygen quenching of fluorescence, whereas TruFluor® pH sensors are based on dual lifetime referencing. There is no detectable photo-induced drift in a 3-week period sampling at 2-second intervals for either of these sensors.

Transmitters: External or Blade

Trufluordo Exttransmitter

TruFluor® External Transmitter

The TruFluor® DO external transmitter is a remote pipe or panel mounted instrument which is plug-and-play with any TruFluor® DO SmartReader. All external transmitters allow automatic entry of calibration and traceability data from the sheath SmartChip and provide diagnostic information. Output signals for DO and temperature are all analog (4 to 20 mA).

Trufluordo Transmitter

TruFluor® Blade Transmitter

TruFluor® digital transmitter blades are mounted in a Finesse bioreactor controller and do not have a display or keypad. All blades provide automatic input of calibration data from sheaths containing a SmartChip and provide detailed diagnostics to the bioreactor control system. The Virtual Transmitter module in Finesse TruBio® software allows full access to all of the blade’s functions, simulates a physical transmitter, and adds alarms and connectivity monitoring capability.

Smart Sensor Loop Validation

Trufluordo Validator

DO Loop Validator

TruFluor® DO validators are designed to test the overall function of a TruFluor® sensor loop. The transmitter, the cable, and the reader are tested as a system to determine the efficacy and veracity of the optical phase measurements. The validator employs a stable light source to simulate an optical measurement of DO. This signal is detected by the TruFluor® reader and results in an equivalent DO value measured by the transmitter.

TruFluor® DO Single-Use Sensor

Environmental

Operating Temperature 4°C to 45°C / 39.2°F to 113°F
Operating Pressure (Typical) 1 bar / 14.7 psi
Storage Temperature 0°C to 60°C / 32°F to 140°F
Storage Relative Humidity 10% to 90% (non-condensing)
Altitude 12192 m / 40000 ft
Materials USP Class VI and 316L EP Stainless Steel

Performance

Sample Rate Settings 1 Second to 30 Minutes
Measuring Range 0 to 52.5% O2 / 0 to 250% air-sat
Limit of Detection 0.03% O2
Accuracy @25°C: ±<1% at 20.95% O2
Response Time (T90. Agitated) < 40 seconds
Calibration Pre-calibrated (SmartChip) 1-point standardization
or 2-point calibration optional
Gamma Radiation Standard range: 26 to 38 kGy (for radiation doses
outside this range contact Finesse)
Cross Sensitivity Organic solvents (e.g. toluene, acetone, benzene,
methylene chloride), sulfur dioxide and chlorine gases.
Temperature Accuracy ±0.25°C between 4°C and 45°C
±0.15°C between 15°C and 40°C
Temperature Precision ±0.1°C

 

TruFluor® DO Validator Specifications

Environmental

Operating Temperature 15°C to 35°C / 59°F to 95°F
Storage Temperature 0°C to 65°C / 32°F to 149°F 
Storage Relative Humidity 0% to 100% (non-condensing)
Altitude 12192 m / 40000 ft
Materials USP class VI and 316L EP stainless steel
Rating NEMA 4X

Performance

Sample Rate Settings Should be set at 1 second during the test
Measuring Range 0% to 100% Sat
Measure of Reference 0, 25, 50, 75, 100
Accuracy (at 20°C) ± 1% Sat for all DO settings
Calibration Pre-calibrated at factory – NIST traceable method

 

TruFluor® DO External Transmitter Specifications

Physical Description

Case Material ABS
Rating NEMA 4X
Dimensions (H x W x D) 157 x 157 x 103 mm / 6.2 x 6.2 x 4.0 in.
Mounting / Conduits Remote, panel or pipe mounted (kit included)
Pg 13.5 conduit fittings accepted
Display 4 line, 20 Character Display, Character Height of 4.8 mm
Weight / Shipping Weight 1 kg / 1.5 kg (2 lb / 3 lb)

Specifications

 
Power Supply 24 VDC @ 150 mA
Signal Outputs (Isolated 4 to 20 mA) 1x DO / 1x temperauture
Output Accuracy ±0.05 mA
Transmitter Diagnostics LED failure, 4 to 20 mA loop errors, or zeroing errors
Calibration Based on NIST traceable standards. Entered
automatically using SmartChip; 1- or 2-point
calibration optional
RFI/EMI EN-61326
Operating Temperature 4°C to 45°C / 39°F to 113°F
Storage Temperature -20°C to 70°C / -4°F to 158°F
Relative Humidity 10% to 90% (non-condensing)

 

TruFluor® DO Blade Transmitter Specifications

Physical Description

Case Material Aluminum bracket
Rating NEMA 1 (when mounted has same rating as enclosure)
Dimensions (H x W x D) 130 x 35 x 128 mm / 5.1 x 1.4 x 5 in
Mounting Mounted within utility tower (controller enclosure)
Display Virtual transmitter in TruBio software (GAMP5)
Weight / Shipping Weight 0.2kg / 0.4kg (0.4 lb / 0.8lb)

Specifications

Power Supply 24 VDC @ 150 mA
Signal Outputs Digital / 1x DO / 1x temperature
Output Accuracy N/A
Transmitter Diagnostics LED failure, 4 to 20 mA loop errors, or zeroing errors.
Reported directly in Finesse TruBio® control software
Calibration Based on NIST-traceable standards. Entered automatically using SmartChip. Optional 1-point standardization or 2-point calibration. Can be read into TruBio® Software with file transfer
or memory stick.
RFI/EMI EN-61326
Operating Temperature 5°C to 45°C / 41°F to 113°F
Storage Temperature 0°C to 65°C / 32°F to 149°F
Relative Humidity 10% – 90% (non-condensing)
Blade transmitters are only sold to approved OEM customers or as spare parts for Finesse control systems.

TruDO and TruDO Optical Sterilizable Sensors

Sterilizable sensors designed for superior performance.